DNA

Part:BBa_K2878003:Design

Designed by: Chen Li   Group: iGEM18_SSHS-Shenzhen   (2018-09-28)


GLS-shRNA-1 template


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GLS-shRNA-1 template sequence starts with GG, which is for transcription efficiency. GC content is around 50%, its target site is not in the first 75 bases after AUG, which are for the RNAi efficiency. UU overhangs in 3 prime-end, which is for the stability of shRNA. The antisense strand of shRNA is reverse complement with the sequence of its target site.


Source

GLS-shRNA-1 template is designed from the sequence of the target site in Glutathione S-transferase mRNA of Phyllotreta striolata, which is 231-251 after AUG. shRNA contains a sense strand, a loop, and an antisense strand.

References